Pọọpọ wepụ ntụ ntụ na ngwa ya na azụmaahịa nri

Atụmatụ maka Pọọpọ wepụ ntụ ntụ:

1. Usoro ịkpọ nkụ

Atụmatụ ihicha bụ usoro mmịpụta kacha mma maka papain. Ọ na-agụnye ịgbakwunye ihe mgbakwunye na pọọpọ mash ma emesịa ikpughe mash ahụ na centrifugation. A na-akpọnwụ nke na-esote supernatant na broiler ikuku na-ekpo ọkụ na okpomọkụ nke 55 ~ 60°C iji nweta ntụ ntụ kemịkalụ siri ike. Ka o sina dị, usoro a adịghị enye aka maka ichekwa mmegharị kemịkalụ, na adịghị mma nke ogige ahụ e kere eke dị ntakịrị.

2. Usoro mmiri ozuzo Tannin

N'ime usoro mmiri ozuzo tannin, a na-enweta ikike pọọpọ site na centrifugation ma mesịa jikọta ya na nhazi tannin. A na-agwakọta ngwakọta a na-adịgide adịgide iji rụzuo ihe kachasị mma nke tannin. Mgbe oge edozi, a na-akwado mgbagwoju anya nke nchikota. A na-agbanwe pH uru nke mgbagwoju anya, ma emesịa, a na-akpọnwụ ma kpughee ya na ọgwụgwọ agụụ iji nweta ihe kemịkal ahụ. Atụmatụ a na-ewepụta ihe na-akpali akpali na mmegharị dị elu mana ọ nwere obere mmịpụta ma nwee ike ibute mmetọ eke.

3.Ultrafiltration Technique

A na-eji mmepụta ihe ọhụrụ Ultrafiltration kewapụ papain. Papain papaya nwere n'ozuzu oke irighiri ihe, ya mere, n'oge ultrafiltration, nnukwu atom na-ejide oyi akwa, ebe ihe ndị ọzọ dị obi umeala irighiri ihe na mmiri na-ekwe ka ịgabiga, na-arụ ọrụ detachment na ọcha. Atụmatụ a na-enye papain na omume ọma dị elu, agbanyeghị, ọ nwere ike ime ka ọsọ ultrafiltration belatara n'ihi nchịkọta nke nnukwu ihe dị n'elu oyi akwa.

4. Atụmatụ mmiri ozuzo nnu

Atụmatụ mmiri ozuzo nnu na-agụnye ịgbakwụnye etiti kwesịrị ekwesị nke ammonium sulfate iji zaa maka nhazi ihe ike siri ike yana ịgbanwe ụkpụrụ pH nke nhazi ahụ. A na-ekewapụ nhazi ahụ ma mee ngwa ngwa iji nweta ezigbo ihe na-akpali akpali. Usoro a na-amịpụta protein ndị nwere omume ọma dị elu n'ozuzu ya, dabara maka ịmepụta mgbazi. N'ọnọdụ ọ bụla, mgbasawanye nke ọtụtụ nnu n'oge usoro mmịpụta na-ewuli ihe mkpofu na ihe na-akpali akpali ma na-ebelata mmegharị papain, na-eme ka ọ bụrụ ihe ọ bụla ma ọ bụghị usoro nchịkọta kachasị mma.

5. flocculation Strategy

Atụmatụ ịkwọ ụgbọ mmiri ahụ na-ezo aka n'ịgbakwụnye ihe ndị nwere ike iji papain kechie ụlọ ndị na-adịghị edozi ahụ na ntinye rọba pọọpọ. A na-emeziwanye ngwakọta ahụ iji nweta kemịkalụ. Usoro a nwere mkpụrụ protein dị elu mana ọ na-eweta mbelata nke ọma nke papain nke ewepụrụ n'ihi ọnụnọ nke mmetọ.

6. Ultrasonic mmịpụta Strategy

Dị ka usoro mmịpụta mbụ maka papain, usoro mmịpụta ultrasonic na-eji mmetụta cavitation na-emepụta site na ebili mmiri ultrasonic iji meziwanye penetrability nke mgbidi cell, na-arụ ọrụ na mbata nke ihe intracellular ma na-ewusi mgbanwe mgbanwe n'ime na n'èzí sel. Na ultrasonic mmịpụta nwere ike ime ka elu mmịpụta ọnụego. Usoro a na-agụnye iji ultrasound na-agwọ mash pọọpọ ọhụrụ, nke ejiri centrifugation na-azụ ya, nzacha, na n'ikpeazụ na-achịkọta ihe atụ kemịkalụ site na ike. Nhazi kemịkalụ siri ike nwetara nwere obi ụtọ dị obere ma chọọ ihicha ọzọ.

7. Usoro ihe nkiri chromatography Proclivity

chromatography ihe nkiri ele mmadụ anya n'ihu bụ mwepu ọnụ na usoro nhicha nke na-eme ka nkebi na-amasị mmasị na nkewa oyi akwa, nwee àgwà nke abụọ. Usoro a nwere ike kewapụ nke ọma ma mee ka papain dị ọcha, yana oke mgbasa ozi dị elu, ogbugbu chromatographic mara mma, yana oke adịghị mma.

8. Usoro mmịpụta nke okpukpu abụọ nke mmiri mmiri

Usoro mmịpụta nke okpukpu abụọ nke mmiri na-eji ihe dị iche iche dị na ngwungwu ihe dị n'ime usoro mmiri abụọ iji wepụ papain. Usoro a na-arụpụta nsonaazụ mmịpụta dị ukwuu, na-enwe mmegharị ngwakọta kachasị ama na mkpụrụ. Ọ na-enweta uru doro anya, dịka ọmụmaatụ, ọrụ na-adịghị akwụsị akwụsị, enweghị nrụpụta nke mmetọ ma ọ bụ ihe mgbaze sitere n'okike, ọnụ ahịa nhazi dị ala, yana nkà dị elu maka mmịpụta papain.

Ojiji nke pọọpọ wepụ ntụ ntụ na azụmaahịa nri:

1. Dị ka anụ anụ

Site n'ịrụ ọrụ na atụmanya maka ntụsara ahụ kwa ụbọchị, ndị mmadụ n'otu n'otu nwere ihe dị elu dị mkpa maka ekpomeekpo anụ. Mgbasawanye nke ndị na-eri anụ nwere ike izu ike ma mee ka elu anụ ahụ dị ọhụrụ. Papain bụ protease cysteine ​​a na-ejikarị na ụlọ ọrụ na-ahụ maka nri iji mepụta anụ nro. Papain nwere ike mebie eriri akwara na anụ ahụ jikọtara nke ọma, na-ekewa myofibrils na collagen n'ime peptides na amino acid dị ntakịrị, na-ebute elu dị nro ma dị ụtọ. Ike ike Papain na-eme ka ọ bụrụ ihe ezi uche dị na ya iji na-edozi nri na-ekpo ọkụ, na-eme ka anụ ahụ dịkwuo mma n'oge isi nri.

2. Na-emepewanye ogo Brew

Mgbasawanye nke papain na-ebuwanye ibu nwere ike ịrụ ọrụ na àgwà ya. Papain na-eme ka protein dịkwuo ukwuu, na-akpali nhazi nke peptides cysteine ​​protease ọzọ na amino acids. Usoro a na-aga n'ihu na ịdị mma nke ime biya n'oge oyi. Na mgbakwunye, ọ na-akwalite uto nke ime biya ma na-arụ ọrụ n'ịdị mma ya n'ozuzu ya.

3. Mpempe akwụkwọ nri Conditioner

Ịtinye papain na mpịakọta bred na ihe ndị e ghere eghe nwere ike ime ka ntụ ọka ahụ sie ike, na-eme ka achịcha ahụ na-agbagharị ma na-eme ka ọdịdị ya dị elu na elu dị larịị. Ọ na-ebelata itinye shuga na abụba n'ahịa dị iche iche a kwadebere, na-eme ka ejiri ya mee ihe n'ụzọ zuru ezu na okike ha.

4. Ịmepụta ihendori

Enwere ike iji Papain mebie ihe iko achịcha lager, na-eweta mwepụ ihe iko achịcha bara ụba na mpaghara ike maka a. Site na nkwadebe, enwere ike ịme ya nke ọma ka ọ bụrụ nri, na-emelite uru yist bara uru.

5. Mepụta nkwalite ndị dị mma

Enwere ike iji Papain mepụta nkwalite na-edozi ahụ. Ọ na-akwado nnabata na iri nri ma enwere ike iji ya mee mbadamba mbadamba eriri afọ iji rụọ ọrụ na ahụ erughị ala afọ. Ọzọkwa, papain nwere ike ikewapụ protein ndị a na-achọpụta na osisi na ihe ndị e kere eke n'ime mmiri mmiri dị mma.

6. Mepụta isi mmalite nri anụ ụlọ

Enwere ike iji Papain na-emeso ụdị nri anụ ụlọ, dị ka nri canine na nri anụ ọhịa, na-eme ka elu ahụ dị nro ma dị mfe nhazi na ntinye. Ọzọkwa, papain nwere ike ịchụpụ ikpuru, na imepụta ya ka ọ bụrụ ihe ọṅụṅụ pọọpọ nwere ike inye aka n'iwepụ nematodes na traktị nri nri nke ihe ndị e kere eke nke ọma.

ọgwụgwụ:

Pọọpọ wepụ ntụ ntụ A na-eji ya eme ihe n'ahịa nri, ọkachasị n'ijikwa anụ, lager, na sauces. Ka atụmanya ndị mmadụ n'otu n'otu maka ntụsara ahụ kwa ụbọchị na-akawanye mma, njikwa nri na nri aghọọla nchegbu ndị bụ isi, na-eme papain ka ọ bụrụ ihe dị mkpa na-edozi ahụ nke ọma na ahụike yana ohere ọganihu ahịa dị oke egwu. Iji gboo mkpa papain dị n'ụdị dị iche iche, ọkachasị na njikwa nri na ụlọ ọrụ mmeju ahụike, a ga-eduzi nyocha nke elu ruo ala na ụzọ nzacha na nzacha maka papain iji kewapụ papain na enweghị mmechuihu dị elu.

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Isi mmalite: Wei Zhaoying, "Mmepụta Papain na Ngwa ya na Azụmahịa Nri."